Coding

Part:BBa_K2638001

Designed by: Erika Schneider   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)
Revision as of 23:20, 2 September 2018 by Registry (Talk | contribs)

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CopC coding sequence

CopC is a gene that encodes for a soluble protein within the periplasmic space(β-barrel structure) with a highly specific Cu(I) and another highly specific Cu(II) binding site. It is part of the CopABCD gene cluster. CopC in Pseudomonas syringae pathovar tomato promotes Cu(II) uptake into the periplasmic space. It was used a homologue from Pseudomonas brassicacearum 3Re2-7 13. Together with CopD (BBa_K2638002) it promotes copper uptake from outside the cell into the cytoplasm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 304
    Illegal AgeI site found at 358
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 246


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Categories
Parameters
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