Other

Part:BBa_K2560002:Design

Designed by: Daniel Stukenberg   Group: iGEM18_Marburg   (2018-08-13)
Revision as of 12:51, 13 August 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Phytobrick Entry Vector with GFP dropout


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1035
    Illegal SapI.rc site found at 193


Design Notes

More information to come


Source

The Phytobrick Acceptor Vector BBa_P10500 was used as a Template. LacZ cassette was replaced by sfGFP cassette which was PCR amplified from pYTK001 (Lee et al. 2015) and cloned into the vector with Gibson Assembly.

References