Measurement

Part:BBa_J100430

Designed by: Tatianna Travieso   Group: Campbell M Lab   (2018-06-28)
Revision as of 17:48, 28 June 2018 by Registry (Talk | contribs)

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T7 Promoter with Downstream Gal4 Binding Site in pClone mScarlet

Gal4 binding site inserted downstream of a T7 promoter consensus sequence (J100403) that will produce RFP when inserted into pClone Red (J119137). When present the Gal4 protein will bind to the site downstream of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 912
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 912
    Illegal BamHI site found at 858
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 912
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None