Composite

Part:BBa_M50420:Design

Designed by: Sruthi Raguveer   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 03:19, 12 June 2018 by Raguveer (Talk | contribs)


Mosquito Larvae Insecticide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 188
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized the amino acid sequence for E. coli. We did not make any other modifications from the source sequences.


Source

The source is previously established parts and the protein from uniprot cry11Aa.

Promoter:

RBS:

Leader Sequence:

Gene:

6x-His Tag:

Terminator:


References