Composite
Part:BBa_M50437:Design
Designed by: Lillian Zhu, Kelsie Wysong, Eva Frankel Group: Stanford BIOE44 - S11 (2018-04-25)
2,3-DHB Biosynthesis Construct, with entC and ahpC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 234
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 234
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 234
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 234
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 234
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our plasmid contained two distinct coding sequences, separately delineated with ribosome-binding sites.
Source
T5 promoter: BBa_K784005