Composite
pHlash

Part:BBa_M50440:Design

Designed by: Adam C Elliott   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 17:29, 11 June 2018 by Aelliott (Talk | contribs)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 759
    Illegal AgeI site found at 1648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1500
    Illegal BsaI.rc site found at 1194
    Illegal SapI.rc site found at 1513

Design Notes

Source

GAL1 promoter sequence: BBa_J63006

pHlash sequence: pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH, PLOS (BBa_M50440)

Terminator sequence: BBa_K1462070

References

https://parts.igem.org/Part:BBa_J63006

https://parts.igem.org/Part:BBa_K1462070

Zhang, Yunfei et al. “pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH.” Ed. Valentin Ceña. PLoS ONE 7.8 (2012): e43072. PMC. Web. 9 May 2018.