![](https://parts.igem.org/images/partbypart/icon_signalling.png)
Signalling
Part:BBa_K2271016
Designed by: Jason Marcel Müller, Manuel Lentzen Group: iGEM17_Cologne-Duesseldorf (2017-10-27)
Revision as of 03:52, 2 November 2017 by MarvinHubert (Talk | contribs)
PTS1 variant P*
The peroxisomal targeting signal 1 (PTS1) is a protein localization signal which is recognized by the Pex5 receptor protein. Cargo proteins which contain this C-terminal PTS1 tag were imported into the peroxisomes in dependence to Pex5 interaction. The native PTS1 sequence contains three amino acids (SKL motive; Serine-Lysine-Leucin).
In our project, we designed an artificial PTS1 peptide sequence which is recognized by our modified Pex5 (BBa_K2271067) in order to provide a fully orthogonal peroxisomal protein import machinery in yeast strains deficient for the natural Pex5 gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |