Part:BBa_K2271016

PTS1 variant P*

The peroxisomal targeting signal 1 (PTS1) is a protein localization signal which is recognized by the Pex5 receptor protein. Cargo proteins which contain this C-terminal PTS1 tag were imported into the peroxisomes in dependence to Pex5 interaction. The native PTS1 sequence contains three amino acids (SKL motive; Serine-Lysine-Leucin).
In our project, we designed an artificial PTS1 peptide sequence which is recognized by our modified Pex5 (BBa_K2271067) in order to provide a fully orthogonal peroxisomal protein import machinery in yeast strains deficient for the natural ===Results=== [[Image:Artico_r19lvl2col.png|thumb|center|'''Figure 1:'''PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1* P* and the PEX13 membrane anchor fused to mRuby.]] Pex5 gene.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]