Coding

Part:BBa_K2271012

Designed by: Eric Behle   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 23:31, 1 November 2017 by Pauline tot (Talk | contribs)


Pex34 with galactose inducible promoter

This part contains the coding sequence for the peroxin Pex34 with the galactose inducible promoter GAL1 and the terminator ScADH1. The sequence is codon-optimized for S. cerevisiae.


Usage and Biology

Pex34p is a peroxisomal integral membrane protein that can act both, independently and in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 971
    Illegal BglII site found at 533
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 149
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None