Regulatory
VirB1

Part:BBa_K2433004:Design

Designed by: Evan Gibbard   Group: iGEM17_British_Columbia   (2017-10-25)
Revision as of 06:20, 1 November 2017 by Eg96 (Talk | contribs) (→‎References)


VirB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 118


Design Notes

The VirB1 promoter was designed by taking the promoter region of virB1 from the pTiC58 - a Ti plasmid from Agrobacterium Tumefaciens. The nucleotide at position 339 was changed to a T on the coding strand to eliminate an XbaI cutsite.

Source

Upstream regulatory region of virB1 from pTiC58.

References

AE007871.2