RNA

Part:BBa_K2379004:Design

Designed by: Ramit B   Group: iGEM17_SVCE_CHENNAI   (2017-10-01)
Revision as of 19:16, 1 November 2017 by Aditi (Talk | contribs) (→‎Design Notes)


RNA Thermometer Optimised for Bacillus Subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been obtained by replacing the Shine Dalgarno sequence of E coli to that of Bacillus Subtilis, present in the RNA Thermometer BBa_K115001. The sequence was fed to RNA VIENNA FOLD and it was found that the structure of the RNA thermometer for Bacillus Subtilis was very similar to that of E Coli with nearly equal thermodynamic free energies.

Source

nil

References

1. Band, L. and Henner,D. J.: Bacillus subtilis requires a “stringent” Shine-Dalgarno region for gene expression.DNA 3 (1984) 17-21.