Part:BBa_K2232003

C125-TupA

This part is the coding sequence (CDS) of a cytoplasmic protein TupA (GenBank: BAB07375.1), which catalyzes the conversion of glucuronic acid and L-glutamic acid to polyglucuronic acid and poly-γ-L-glutamic acid.

Usage and Biology

Gene tupA was cloned from the chromosomal DNA of facultative alkaliphilic strain Bacillus lentus C-125. The primary translation product of this gene, TupA, is likely a cytoplasmic protein(57.3 kDa) consisting of 489 amino acid residues.It was demonstrated that TupA is involved in the synthesis of TUP,a copolymer of polyglutamic acid (PGlu) and polyglucuronic acid (PGlcU),which is one of major structural components in the cell wall of the Bacillus lentus C-125 and can neutralize the extracellular hydroxyl. A mutant defective in TUP synthesis grows slowly at alkaline pH,indicating this gene plays a key role in pH homeostasis and increases the alkali resistance of Bacteria.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 969
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 789
  Illegal BsaI.rc site found at 1181