Part:BBa_K2213012
LacUV5_EutS_Tet_EutMN
LacUV5_EutS_Tet_EutMN is a composite of parts https://parts.igem.org/Part:BBa_K2213000 and https://parts.igem.org/Part:BBa_K2213001.
Figure 1: Schematic overview of LacUV5_EutS_Tet_EutMN (BBa_K2213012)
Characterisation
This part was expressed alongside EutLK-Low-PduD-mCherry-PPK (https://parts.igem.org/Part:BBa_K2213013) to form EutSMNLK+Low_PduD+PPK which was characterized as follows.
A 24 hour induction was DAPI stained to determine microcompartment formation and tag localisation.
Figure 2: Fluorescence microscopy images of promoter-PduD associated mCherry and DAPI stained polyphosphate.
As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1260
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4125
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3451
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