Composite

Part:BBa_K2389010

Designed by: Rochelin Dalangin   Group: iGEM17_UAlberta   (2017-10-26)
Revision as of 19:32, 26 October 2017 by Registry (Talk | contribs)

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pT8O-LZ

This part is the redesigned BACTH plasmid, containing both T18 and T25 subunits each fused to a leucine zipper, under the control of a constitutive promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 633
    Illegal BamHI site found at 1605
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 141
    Illegal NgoMIV site found at 551
    Illegal AgeI site found at 357
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None