Composite
Part:BBa_K2457005
Designed by: Maria Astolfi, Giovanna Maklouf, Juliana Vitoriano Group: iGEM17_Amazonas_Brazil (2017-09-23)
Standardized pBAD_Cas9 device
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Usage and Biology
Design
Figure 1: BBa K2457005 circuit
Characterization
Sequencing
Figure 2: Sequencing electropherogram from BBa_K2457005.
Figure 3: Alignment of the designed sequence and our final construction from BBa_K2457005.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1207
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1042
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1024
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Categories
Parameters
None |