Regulatory

Part:BBa_K2365031

Designed by: Tong Cheng   Group: iGEM17_NAU-CHINA   (2017-10-04)
Revision as of 00:43, 2 November 2017 by Tinaqin (Talk | contribs)


TDH1 promotor

TDH1 Yeast promoter Constitutive expression ; Glyceraldehyde-3-phosphate dehydrogenase,a weak constitutive promoter,which catalyzed 3-phosphoglyceraldehyde oxidation (dehydrogenation) and phosphorylation.As the center of glucose metabolism,TDH1 plays an important role in glucose metabolism. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 431
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


PCR result

TDH1 TDH1-cyc1 NAU-04.jpeg
启动子全部.jpeg

Figure2.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.

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