Composite

Part:BBa_K2365508

Designed by: HangYu Duan   Group: iGEM17_NAU-CHINA   (2017-10-20)
Revision as of 22:37, 1 November 2017 by Tinaqin (Talk | contribs)


Bax induced part

This part is used to as the key part of our biosafety device. Coding sequence of Bax protein is linked to the Gal1 promotor. It could be inserted to your device as kill switch when your chassis fungal is yeast(in the most broadly yeast).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


图片3.png

The 579bp fragment was cloned under the GAL1 promoter, followed by CYC1 terminator. The pYES2 vector is designed for native expression of protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2µ origin for high-copy maintenance. After verifying the right constructs by restriction enzyme digestions and sequencing, the plasmids were transformed into S. cerevisiae SEY6210.

[edit]
Categories
//biosafety
Parameters
None