Composite

Part:BBa_K2440009:Design

Designed by: Chushu Zhu   Group: iGEM17_NUDT_CHINA   (2017-10-27)
Revision as of 23:26, 27 October 2017 by Registry (Talk | contribs)

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CMV promoter ->Flag tag -> hAgo2-> SV40 PA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3288
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 717
    Illegal NheI site found at 3425
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1863
    Illegal XhoI site found at 2773
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3288
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3288
    Illegal NgoMIV site found at 726
    Illegal AgeI site found at 2561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1423


Design Notes

aa


Source

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References