Device

Part:BBa_I742132

Designed by: Caroline Dahl   Group: iGEM07_Edinburgh   (2007-10-22)
Revision as of 18:38, 22 October 2007 by Registry (Talk | contribs)

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Division-PoPper: Cell division --> PoPS

This device gives PoPS-output on cell division. The DNA flanked by the opposing repeats of the dif-site is inverted and allows transcription of both any downstream element and a repressor which, upon sufficient build-up, switches off the promoter which controls downstream transcription. Inversion only occurs when the XerCD complex is activated, and this only occurs at cell division.

Therefore this device produces a short burst of PoPS as a function of cell division. Any added downstream elements will likewise be activated, briefly, as a function of cell division.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None