Composite

Part:BBa_K2423008

Designed by: Oscar Broström   Group: iGEM17_Uppsala   (2017-10-01)
Revision as of 10:24, 27 October 2017 by Rixina (Talk | contribs)


UGTCs2 with BBa_J04500

UGTCs2 is an enzyme that catalyzes the conversion of crocetin to crocin. This is the third and final step in the zeaxanthin to crocin pathway. In nature this enzyme can be found in Crocus Sativus. Crocus Sativus is the plant where saffron is harvested from.

The gene of interest in this part is regulated by BBa_J04500. This implies that it has to be induced by lactose or any of its closely related derivates, such as IPTG.

This BioBrick is an improved version of BBa_K1033112 and a slightly modified version of BBa_K2423002 (different promoter and RBS).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 758
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 931
    Illegal AgeI site found at 1286
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None