Part:BBa_K2384003

DsbA+MBP(Pb)

the periplasm display protein Dsba-MBP, which binding the lead of the enviroment. Disulfide bond formation protein A (DsbA) is a powerful oxidative protein of the thioredoxin family. DsbA can help protein to fold correctly both in vivo and in vitro 。The C-terminal fusion protein of the maltose-binding protein can be efficiently and rapidly output into the periplasm as a key component of the expression of the metal-binding peptide of the cyclin.

Our sequence optimization is based on the sequence provided by Peking University's IGEM Wiki in 2010.It is more suitable for expression in Bacillus

Usage and Biology

Optimization Report

Gene Name:DSBA-MBP(Pb)

Expression System:Bacillus megaterium

Gene Length:963 (bp)

1.Codon Used Adjustment The best value is 1 for sequence optimization.

Figure 1:Before Codon Adjustment

Figure 2:After Codon Adjustment

2.Codon Used Distribution Show the relative codon used distribution

Figure 3:Before Optimization

Figure 4:After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Figure 5:Before Optimization

Figure 6:After Optimization

Figure 7:The expression of DsbA+MBP(Pb) detected using SDS-PAGE

Figure 8:Plasmid digested by EcoRI-PstI

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 255
  Illegal BglII site found at 267
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]