Composite

Part:BBa_K2324012:Design

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-16)
Revision as of 20:19, 16 October 2017 by Registry (Talk | contribs)

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pJ23100_FimOp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 931
    Illegal AgeI site found at 962
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The operon sequence had to be divided up for synthesis (due to its length) before being ligated together. The natural transcriptional machinery of the operon had to be preserved throughout this cloning process.


Source

Part BBa_K1850012 was the source for the sequence of the operon for the CDS. Parts BBa_B0034 and BBa_B0015 were the ribosome binding site and terminator, respectively, found on the registry. The source for the J23100 promoter was part BBa_J23100.

References