Composite

Part:BBa_K2324015

Designed by: Karolina Kostrzynska   Group: iGEM17_Exeter   (2017-11-01)
Revision as of 20:57, 1 November 2017 by Karolina8 (Talk | contribs)


Arabinose inducible fim operon

description of the arabinoise inducible fim operon.

This part contains the fim operon under the control of an inducible arabinose promoter. The operon contains six Fim proteins, including FimA, FimI, FimC, FimD, FimF and FimG. When co-transformed with FimH constructs from our project, which have rhamnose-inducible promoters, modified type I pili may be produced. The production of the operon must be initiated by inducing the culture overnight at 0.6 OD at 2% arabinose.


Figure 1 We transformed a plasmid containing the fim operon under the control of the P_Ara promoter. It exhibits flagella on their cell surface, but the electron micrograph shows a suggestion of pili.


This suggests that the P_Ara construct was successful in synthesising pili (minus FimH)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 2743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1026
    Illegal AgeI site found at 1057
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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