Coding

Part:BBa_K2515000:Design

Designed by: Slavil Peykov   Group: iGEM17_Bulgaria   (2017-10-26)
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KillerOrange CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 5' end nucleotide sequence was codon optimized (no effect on AA sequence) to avoid formation of stable secondary structures. After this modification one can easily design primers for this part without scars.


Source

gBlock synthesis. Coding sequence comes from the following article: "Genetically Encoded Photosensitizer Activated by Blue and Green Light" PLoS One. 2015 Dec 17;10(12):e0145287. doi: 10.1371/journal.pone.0145287. eCollection 2015.

References