Composite

Part:BBa_K2324006

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-16)
Revision as of 18:29, 1 November 2017 by DanBarber (Talk | contribs)


pRha_FimH_225sfGFP

This part produces a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should initiate pilus biosynthesis when co-transformed with a plasmid containing the fim operon .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None