Coding
Part:BBa_K2446055:Design
Designed by: Yijie Pan Group: iGEM17_Fudan (2017-10-26)
SynNotch-anti-EGFP-tTA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 381
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2691
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1606
Design Notes
This is the core part of the SynNotch. With synthetic extracellular recognition domain (SynECD, e.g. scFv),and the synthetic intracellular transcriptional domain (SynICD, e.g. SynTF), the other two parts of SynNotch which iare changeable by needs. The SynECD and SynICD can assembly with Notch core by fusion protein technique.
Source
RNA was extracted from mouse whole kidney, and cDNA was produced by commercial kit following corresponding protocol, and used as the template of PCR.