Coding

Part:BBa_K2446055:Design

Designed by: Yijie Pan   Group: iGEM17_Fudan   (2017-10-26)
Revision as of 11:03, 26 October 2017 by Registry (Talk | contribs)

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SynNotch-anti-EGFP-tTA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2691
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1606


Design Notes

This is the core part of the SynNotch. With synthetic extracellular recognition domain (SynECD, e.g. scFv),and the synthetic intracellular transcriptional domain (SynICD, e.g. SynTF), the other two parts of SynNotch which iare changeable by needs. The SynECD and SynICD can assembly with Notch core by fusion protein technique.


Source

RNA was extracted from mouse whole kidney, and cDNA was produced by commercial kit following corresponding protocol, and used as the template of PCR.

References