Composite

Part:BBa_K2448033:Design

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 11:11, 1 November 2017 by Brumake (Talk | contribs)


D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum under the control of pTacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

pTacI promoter (BBa_K864400) + RBS (BBa_K2448019) + D-psicose 3-epimerase from Clostridium cellulolyticum coding sequence (BBa_K2448021)

Source

DNA synthesis

References

[1] Mu W, Chu F, Xing Q, Yu S, Zhou L, Jiang B. Cloning, expression, and characterization of a D-psicose 3-epimerase from Clostridium cellulolyticum H10. J Agric Food Chem. (2011) 59, 7785-7792.