RNA

Part:BBa_K2515002:Design

Designed by: Slavil Peykov   Group: iGEM17_Bulgaria   (2017-10-27)
Revision as of 08:22, 27 October 2017 by Registry (Talk | contribs)

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gRNA expression vector for use with Cas9 or dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 64
    Illegal BsaI.rc site found at 38


Design Notes

This part was inspired by the following publication: "CRISPR interference (CRISPRi) for sequence-specific control of gene expression" doi:10.1038/nprot.2013.132


Source

This part was made as gBlock fragment by IDT.

References