Composite

Part:BBa_K2384015:Design

Designed by: Fangfei Niu   Group: iGEM17_FAFU-CHINA   (2017-10-27)
Revision as of 17:46, 1 November 2017 by Ocean (Talk | contribs)


Repressor gene xylR+Strong PxylA promoter+RBS+Lpp-Omp-MBP(Pb)+His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CDS sequence optimization in BBa_K538004 is better suited for expression in Bacillus megaterium


Source

This gene is a cold resistant gene from the Arctic cryophile Polaribacter irgensii from BBa_K538004 .

References