Regulatory

Part:BBa_K2286005

Designed by: Licong He   Group: iGEM17_UESTC-China   (2017-10-20)
Revision as of 16:10, 1 November 2017 by Tedemin (Talk | contribs) (→‎Characterization)


PYK10-1457：a seedling and root specific promoter

This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter, PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR. The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that PYK10-1457 is an excellent root-specific promoter.

Fig.1 RT-PCR of CKX3 activated by PYK10-1457.


References

1.Nitz, I., et al., Pyk10, a seedling and root specific gene and promoter from Arabidopsis thaliana. Plant Sci, 2001. 161(2): p. 337-346.


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