Coding

Part:BBa_K2201411

Designed by: Laura Schlueter   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)
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Tyrosyl synthetase library plasmid from M. jannashii with mRFP as optical controle for a selection

Tyrosyl tRNA synthetase from Methanococcus jannashii in pSB1C3, designed as library plasmid for the randomerization of certain positions and following selection cycles for the incorporation of non-canonical amino acids or unnatural bases. In addition to BBa_K2201400, the synthetase contains an mRFP (BBa_J04450)in the position of the synthetases binding pocket. When generating the library with randomerized short double stranded DNA, the mRFP serves as optical controle if the randomerized DNA sequence incorporateded or not.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1275
    Illegal AgeI site found at 1387
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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