Composite

Part:BBa_K2378006

Designed by: Muhammad Farhan Maulana, Marvin Nathanael Iman, Zulhaerana Bahar   Group: iGEM17_ITB_Indonesia   (2017-10-27)
Revision as of 16:18, 1 November 2017 by Marvinnathanael (Talk | contribs)


NhaR-PETase Constitutive Coding Device

This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of NhaR BBa_K1357002 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. This part also use constitutive promoters BBa_J23106.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1002
    Illegal NotI site found at 1911
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 236


[edit]
Categories
Parameters
None