DNA
Part:BBa_K2243010
Designed by: Li Yulong Group: iGEM17_Peking (2017-10-23)
TP901-1 attB_J23119_TP901-1 attP
The constitutive promoter J23119 is between Bxb1 gp35 specific sites and will be inverted by the Bxb1 gp35 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase bxb1 gp35 characterization.
We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 105
Illegal NheI site found at 128 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |