Composite

Part:BBa_K2353001:Experience

Designed by: Julia Leveille, Lauren Hong, Emily Gibson, Gaurav Byagathvalli, Katie Barr, Alyssa Franklin, Christina Lee, Ellie Kim, Kevin Li, Megan Hong, Natalie Shih, Nithik Balachandran, Noora Chandasir, David S   Group: iGEM17_Lambert_GA   (2017-10-17)
Revision as of 15:21, 1 November 2017 by JLeveille (Talk | contribs) (→‎User Reviews)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2353001

User Reviews

UNIQ12bfd1bbf1ad41e6-partinfo-00000000-QINU

BBa_K2353001 Review 1 Not understood jleveille

tsPurpleDAS was ordered and hydrated from IDT and then digested and ligated into 1C3 so that it could be sent for sequencing and then submitted. Running the digest on a gel showed tsPurpleDAS to be the correct length, however when sequenced, we discovered that the DAS degradation tag was not present. It is hypothesized that at some point during the cloning workflow, tsPurple was confused with tsPurpleDAS.
Gel results from a digest of all three tsPurple parts. From top to bottom: BBa_K2353000, BBa_K2353002, BBa_K2353001

;

UNIQ12bfd1bbf1ad41e6-partinfo-00000003-QINU