Part:BBa_K2384010

LacI

Usage and Biology

The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. These genes are repressed when lactose is not available to the cell, ensuring that the bacterium only invests energy in the production of machinery necessary for uptake and utilization of lactose when lactose is present. When lactose becomes available, it is converted into allolactose, which inhibits the lac repressor's DNA binding ability. Loss of DNA binding by the lac repressor is required for transcriptional activation of the operon.

The optimization of this sequence is more suitable for the expression of Bacillus megaterium

Figure 1:Toggle switch.

This part is the combination of other two parts(BBa_K2384010) and (BBa_K2384009) , which could work together as a kill switch that uses a transcription-based monostable toggle design to provide rapid and robust target cell killing, dubbed ‘Toggle switch’

This part has made a condon optimization.

Sequence optimization information:

Expression System: Bacillus megaterium

Gene Length:624(bp)

Show the relative codon used distribution

Figure 4 :Codon Used Distribution after optimization.

Figure 5:GC Content after optimization.

Figure 3: Digested with NotI//.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 115
  
• 1000
  COMPATIBLE WITH RFC[1000]