Composite

Part:BBa_K2477013

Designed by: Asha Kodan, Annie Trang, Cynthia Uzoukwu   Group: iGEM17_UMaryland   (2017-10-26)
Revision as of 23:37, 26 October 2017 by Registry (Talk | contribs)

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Copper Biosensor

This part constitutively expresses the CueR repressor. This repressor then inhibits the expression of RFP from the downstream PcopA promoter. In the presence of copper, the repression of CueR is inhibited leading to greater RFP expression. Fluorescence of this construct has been shown to increase with increasing copper concentration in both a qualitative and quantitative manner.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1237
    Illegal AgeI site found at 1349
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None