Coding
Part:BBa_K2381001
Designed by: Wenqi Huang Group: iGEM17_HZAU-China (2017-10-20)
Revision as of 06:34, 25 October 2017 by Johnson Huang (Talk | contribs)
nMagHigh
nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms.
This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts BBa_K2381017.
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 403
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Categories
Parameters
None |