Part:BBa_K2201010
Construct comprised of PtNTT2, lacO and Cas9
Construct comprised of PtNTT2, lacO and Cas9
Usage and Biology
This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of PtNTT2, please view BBa_K2201004. A characterization of the lacO operon and Cas9 can be found here: BBa_K2201020 and BBa_K2201023.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3356
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3356
Illegal NheI site found at 3115 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3356
Illegal BamHI site found at 5394 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3356
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3356
- 1000COMPATIBLE WITH RFC[1000]
Construct comprised of PtNTT2, lacO and Cas9
Construct comprised of PtNTT2, lacO and Cas9
Usage and Biology
This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of PtNTT2, please view <a href="https://parts.igem.org/Part:BBa_K2201004"> BBa_K2201004</a>. A characterization of the lacO operon and Cas9 can be found here: <a href="https://parts.igem.org/Part:BBa_K2201020">BBa_K2201020</a> and <a href="https://parts.igem.org/Part:BBa_K2201023">BBa_K2201023</a>. Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3356
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3356
Illegal NheI site found at 3115 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3356
Illegal BamHI site found at 5394 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3356
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3356
- 1000COMPATIBLE WITH RFC[1000]
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