Regulatory

Part:BBa_K2309030

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-27)
Revision as of 14:38, 27 September 2017 by Registry (Talk | contribs)

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Switch controller with LacI and TetR

The promoter plac can be repressed by the repressor protein LacR produced by the LacI gene. When LacR reaches a certain concentration, it can stop the promoter plac from transcribing the tetR gene to produce the other repressor protein tetR. Because in Lactococcus lactis, there were originally no natural LacR, the promoter plac is on and is always synthesizing tetR protein to repress the promoter ptet. Therefore, when there is no lacR, tetR can be successfully synthesized and repress ptet, the downstream gene cannot be transcribed. But when LacI in the upstream is transcribed and LacR therefore repress plac, tetR cannot be synthesized anymore, so nothing to repress the ptet results in the downstream gene starts to be transcribed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None