Regulatory

Part:BBa_K305008:Experience

Designed by: Joel Kuiper, Arend Jan Suk   Group: iGEM10_Groningen   (2010-10-18)
Revision as of 09:52, 1 November 2017 by VictorNJ (Talk | contribs)


Applications of BBa_K305008

User Reviews

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victorNJ 

we (AQA_Unesp 2017) made a comparison between srfA and Pveg promoter, which is recognized as being a strong one and is already featured in the registry (BBa_K143012), and we show a relationship of cell growth and expression of GFP. As a tool for measuring the activity of promoters, reporter vectors can be required. Therefore, we chose the luciferase reporter (pBs3Clux-PsrfA), and green fluorescence protein reporter (pBs1C-PsrfA-GFP). For luciferase reporter, we did the percentage of luminescence making the ratio by the point of greatest luminescence and multiplying by 100. Already by GFP, we measured at 535nm for emission and 285nm for excitation. The readings were performed on the Infinite® M200 (Tecan)


For more Details visit our [http://2017.igem.org/Team:AQA_Unesp/Parts]

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