Part:BBa_K2281007
--PdGES--
Introduction
LOCUS GU136162
SIZE 1755 bp
DEFINITION Phyla dulcis geraniol synthase (GES),we optimized the codon to suitable for E.coli, and avoid several restriction enzyme cutting sites(EcoRⅠ, XbaⅠ, SpeⅠ, PstⅠ,BamHⅠ, HindⅢ, KpnⅠ, NocoⅠ, NdeⅠ, NotⅠ, SalⅠ, XhoⅠ)
PdGES stands for Phyla dulcis geraniol synthase (GES) mRNA which is an enzyme which bolsters the production of geraniol. Through the MVA or DXP pathway, Geranyl Diphosphate (GPP) can be produced. Then PdGES helps convert GPP to geraniol.
The diagram shows the complete pathway of the production of citronellol.
胶图:
From the diagram above we can see gene GES has been ligated to the vector pB1SC3 properly.
Sources
SOURCE Phyla dulcis
ORGANISM Phyla dulcis Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Verbenaceae; Lantaneae; Phyla.
From Phyla dulcis which is a species of perennial herb that is native to southern Mexico, the Caribbean (Cuba, Hispaniola, and Puerto Rico), Central America, Colombia, and Venezuela. It is known by several common names, including Aztec sweet herb, bushy lippia, honeyherb, hierba dulce, and tzopelic-xihuitl (Nahuatl). Its buds are also sold as dushi or dulce (sweet in Papiamento and Spanish respectively) buttons.
References
REFERENCE 1 (bases 1 to 1755)
AUTHORS Yang,T., Stoopen,G., Yalpani,N., Vervoort,J., de Vos,R., Voster,A., Verstappen,F.W., Bouwmeester,H.J. and Jongsma,M.A. TITLE Metabolic engineering of geranic acid in maize to achieve fungal resistance is compromised by novel glycosylation patterns JOURNAL Metab. Eng. 13 (4), 414-425 (2011) PUBMED 21296182
REFERENCE 2 (bases 1 to 1755)
AUTHORS Yang,T., Stoopen,G., Yalpani,N., Voster,A., Vervoort,J., de Vos,R., Verstappen,F., Bouwmeester,H. and Jongsma,M. TITLE Direct Submission JOURNAL Submitted (27-OCT-2009) Entomology, Plant Research International, Droevendaalsesteeg, Wageningen 6700AP, The Netherlands
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 184
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1093
None |