Composite

Part:BBa_K2272019

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-25)
Revision as of 02:23, 28 October 2017 by Ew8sk (Talk | contribs)


pVHb-HAO-Cyt

Construction intermediate/device with pVHb promter.


Usage and Biology

A improved version of https://parts.igem.org/Part:BBa_K1067002 and https://parts.igem.org/Part:BBa_K1067004 by combining the two coding sequences, which were suppose to work hand in hand, together. The VHb promoter was inserted to regulate the nitrification circuit expression under certain hypoxic conditions usually found in Sewage tanks. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 484
    Illegal XhoI site found at 2048
    Illegal XhoI site found at 4257
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 500
    Illegal NgoMIV site found at 977
    Illegal NgoMIV site found at 981
    Illegal NgoMIV site found at 2749
    Illegal NgoMIV site found at 3139
    Illegal NgoMIV site found at 3406
    Illegal NgoMIV site found at 3454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 404
    Illegal BsaI.rc site found at 452
    Illegal BsaI.rc site found at 1019
    Illegal BsaI.rc site found at 3179
    Illegal BsaI.rc site found at 4856
    Illegal BsaI.rc site found at 5012


[edit]
Categories
Parameters
None