Regulatory

Part:BBa_K2457000

Designed by: Maria Astolfi, Giovanna Maklouf, Juliana Vitoriano   Group: iGEM17_Amazonas_Brazil   (2017-07-08)
Revision as of 21:15, 31 October 2017 by JulianaNV (Talk | contribs) (→‎Usage and Biology)


araC + pBAD arabinose inducible promoter

Arabinose inducible promoter pBAD forward + araC regulatory reverse gene under control of pC promoter and tryptophan terminator sequence.


Usage and Biology

AraC pBAD electropherogram sequence1.jpeg Figure 1:Sequencing electropherogram from BBa_K2457000.

AraC pBAD alignment.png Figure 2:Allignment of the designed sequence and our final construction from BBa_K2457000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1024


[edit]
Categories
Parameters
None