Coding
Part:BBa_K2433002
Designed by: Tamar Av-Shalom Group: iGEM17_British_Columbia (2017-10-25)
ietAS
ietAS is composite part made up of ietA an ietS the genes encoding the toxin antitoxin system ietAS native to tumor-inducing (Ti) plasmids of Agrobacterium tumefaciens. This part comes from the nopaline type Ti plasmid pTiC58. In combination with the toxin gene, this part has been shown in literature to be important in reducing the transconjugant efficiency for different incoming Ti plasmids as well as contributing to the stability of plasmids harboring this construct.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 737
Illegal BamHI site found at 1758
Illegal XhoI site found at 659
Illegal XhoI site found at 989
Illegal XhoI site found at 3353 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2663
Illegal NgoMIV site found at 3547
Illegal AgeI site found at 3704 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 49
Illegal BsaI.rc site found at 836
Illegal BsaI.rc site found at 1838
Illegal BsaI.rc site found at 3023
Illegal BsaI.rc site found at 3305
Illegal SapI.rc site found at 373
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Categories
Parameters
None |