Device

Part:BBa_K2213002

Designed by: Jessica Burns   Group: iGEM17_Manchester   (2017-08-17)
Revision as of 01:40, 1 November 2017 by Ajfraser (Talk | contribs)


araB_eutLK

The ethanolamine utilisation bacterial microcompartment (BMC) proteins, EutLK (from E. coli) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG (see figure 1).
800px-Lk_operon.png
Fig 1: Schematic of the araBAD_EutLK part (BBa_K2213002)

araBad Promoter

The ara expression system is advantageous as in addition to being induced by arabinose, gene expression can be rapidly downregulated as repression can occur due to catabolite repression in the presence of glucose or by competitive binding of the anti-inducer fucose. Plasmids containing the ara expression system have minimal levels of background expression (Siegele and Hu, 1997).

EutLK






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1300
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1239
    Illegal BamHI site found at 2164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1074
    Illegal AgeI site found at 1775
    Illegal AgeI site found at 1952
    Illegal AgeI site found at 2252
    Illegal AgeI site found at 2315
    Illegal AgeI site found at 2362
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1056



References Siegele, D.A. and Hu, J.C., 1997. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proceedings of the National Academy of Sciences, 94(15), pp.8168-8172.

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