Coding
Part:BBa_K2194000:Design
Designed by: Catherine Dunaway, Anna Guseva Group: iGEM17_Rice (2017-10-23)
chrR6 Chromate Reductase Enzyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 570
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 337
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal BsaI.rc site found at 583
Design Notes
[update]
Source
Escherichia coli genome; mutation Tyr128Asn introduced with PCR site-directed mutagenesis.