DNA

Part:BBa_K2440031

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 01:19, 1 November 2017 by MINXIAO (Talk | contribs) (Usage and Biology)


miR-155 target sequence

It is the target sequence of miR-155, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-155 in an Ago2 dependent manner, that is, knockdown of miR-155 was achieved by transfecting cells with miRNA locker.

Dre-miR155 was firstly found in zebrafish.1

MiR-155-5p are highly expressed in the heart and regulate cardiac development. What’s more, miR-155-5p was overexpressed in trisomic hearts.2

MiR-155 downregulation in NK cells of IA patients impaired IFN-γ production by targeting SOCS1, which may contribute to immune dysfunction during CHB infection. Additionally, baseline miR-155 expression could predict the treatment response to telbivudine therapy.3

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.

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Categories
Parameters
None