DNA

Part:BBa_K2440029

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 01:15, 1 November 2017 by MINXIAO (Talk | contribs) (→‎Usage and Biology)


miR-458 target sequence

It is the target sequence of miR-458, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-458 in an Ago2 dependent manner, that is, knockdown of miR-458 was achieved by transfecting cells with miRNA locker.

Dre-miR-458-5p is a novel RNA founded in zebrafish1. And its function is still unknown.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.

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Categories
Parameters
None