Device
Part:BBa_K2213002
Designed by: Jessica Burns Group: iGEM17_Manchester (2017-08-17)
araB_eutLK
The ethanolamine utilisation bacterial microcompartment (BMC) proteins, EutLK (from E. coli) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG (see figure 1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1300
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1239
Illegal BamHI site found at 2164 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1074
Illegal AgeI site found at 1775
Illegal AgeI site found at 1952
Illegal AgeI site found at 2252
Illegal AgeI site found at 2315
Illegal AgeI site found at 2362 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1056
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Categories
Parameters
None |