Part:BBa_K2440016

miR-511 target sequence

It is the target sequence of miR-511, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker.

Hsa-miR-511-5p was firstly found in human placenta by an integrative approach combining bioinformatics predictions with microarray analysis and sequence-directed cloning. It is located in chr 10 17927113-17927199 and chr 10 18174042-18174128.¹ It has been reported that hsa-miR-511-5p can act as an intracellular mediator of glucocorticoids and TGF-β for the induction of endotoxin tolerance in human monocytes.²

Decreased expression of miR-138-5p and increased expression of miR-147a, miR-147b and miR-511-5p in HCV DLBCL was found to be a poor prognostic factor for HCV-positive DLBCL patients.³

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 4
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 4
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 4
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 4
1000
COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

Reference=

1.Identification of hundreds of conserved and nonconserved human microRNAs. Bentwich I, Avniel A, Karov Y, Aharonov R, Gilad S, Barad O, Barzilai A, Einat P, Einav U, Meiri E, Sharon E, Spector Y, Bentwich Z Nat Genet. 37:766-770(2005). 2.Glucocorticoids downregulate TLR4 signaling activity via its direct targeting by miR-511-5p. Curtale G, Renzi TA, Drufuca L, Rubino M, Locati M. 3.MicroRNA as potential biomarker in HCV-associated diffuse large B-cell lymphoma.Augello C, Gianelli U, Savi F, Moro A, Bonoldi E, Gambacorta M, Vaira V, Baldini L, Bosari S.